hero-image
HOME
hero-image
project-highlight-image

Adesida Laboratory Tissue Engineering Research

hero-image
Aahil Ansari

Project Timeline

Apr 2025 - Sep-2025

OVERVIEW

In the Adesida Laboratory, I contributed to cartilage tissue engineering research by developing automated imaging pipelines, performing histological and biochemical assays, and optimizing 3D bioprinting workflows. I created a Python-based image quantification script that reduced analysis time by 50%, accelerating data processing for brightfield and immunofluorescent microscopy. I conducted tissue culture, embedding, slicing, staining, and imaging to support morphological and biochemical characterization of cartilage constructs. Additionally, I performed RNA/cDNA extraction for RT-qPCR studies and calibrated the Lumen X Gen 3 bioprinter using custom Fusion 360 components to enable reliable PEGDA scaffold fabrication.

HighlightS

  • Built a Python image-analysis tool that cut quantification time for microscopy data by 50%.
  • 🧪 Performed full histological workflows—tissue culture, embedding, slicing, staining, and imaging—for cartilage assessment.
  • 🧬 Executed RNA and cDNA extraction to support RT-qPCR-based transcriptomic analysis.
  • 📊 Ran biochemical assays (DMMB) to quantify proteoglycan and DNA content under mechanical stimulation.
  • ⚙️ Calibrated the Lumen X Gen 3 bioprinter using custom Fusion 360 components for reliable PEGDA scaffold printing.
  • 🔬 Contributed to multi-modal characterization integrating histology, biochemistry, and gene expression data.

SKILLS

PythonHistologyMicroscopyBioprintingFusionRNAStainingAssaysQuantificationTissue
lowinertia
Portfolio Builder for Engineers
Created by Aram Lee
© 2025 Low Inertia. All rights reserved.